
Type:MouseIgM
Applications:IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:Wholeserum-lyophilized
Immunogen:TheTHmoAbrecognizesanepitopewithinthecatalytically-activeC-terminalend-product.ThisC-termproductis~34kDa.(ThisendproductistheresultofapartialdigestionoftheratTHwithchymotrypsin.)

Thehistochemicalantibodyfortyrosinehydroxylase(TH)isamousemonoclonalantibodygeneratedagainstTHthathasbeenisolatedandpurifiedfromratPC12cells. Theantibodyisbelievedtohavewidespeciescross-reactivitybecauseitrecognizesanepitopeinthemid-portionoftheTHmoleculewhereextensivespecieshomologyexists.
ThisTyrosineHydroxylaseantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethods. Theantiserumdemonstratesstronglypositivelabelingofratcatecholamineneuronsystemsusingindirectimmunofluorescentandbiotin/avidin-HRPtechniques. Recommendedprimarydilutionsforthesemethodsareprovidedonthedata-sheet.Thisantibodydoesnotcrossreactwithdihydropterdinereductase,dopamine-B-hydroxylase,phenylethanolamine-N-methyltransferase,phenylalaninehydroxylaseortryptophanhydroxylaseusingWesternblotmethods.
Image:THStainingintheSubstantiaNigra.
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