
Type:MouseIgG
Applications:ICC;IF;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:B;Ch;H;M;P;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid

Ubiquitinisahighlyconservedglobular76aminoacidproteinofabout8.5kDamolecularweight.Ithasanimportantroleinthetargetingofproteinsforproteolyticdegradation.ProteinstobedegradedarecovalentlycoupledtotheC-terminusofubiquitinbymeansofubiquitinligases.Theubiquitinitselfisfrequentlyalsoubiquitinated,producingapolyubiquitinchain.Thepolyubquitinatedcomplexisthenrecognizedbyacomplexofdegradativeenzymeswhichtogetherformtheproteosome.Interestingly,ubiquitinalsobecomescovalentlybondedtomanytypesofpathologicalinclusionsseeninserioushumandiseasestateswhichappeartoberesistanttonormaldegradation,sothatubiquitinantibodiesareveryusefulforstudiesoftheseinclusions.ForexampletheneurofibrillarytanglesandpairedhelicalfilamentsdiagnosticofAlzheimer’sdisease,theLewybodiesseeninParkinson’sdisease,andPickbodiesfoundinPick’sdiseaseareallheavilyubiquitinatedandcanallbereADIlyvisualizedwithubiquitinantibodiesofappropriatespecificity.
Image:MO22152stainingofcerebralcortexofanAlzheimerpatient.Neurofibrillarytanglesanddystrophicneuritesassociatedwithsenileplaquesstainstronglywiththisantibody.Inthecenterisatypicalneurofibrillarytanglecontainingneuron