
Type:RabbitIgG
Applications:ICC;IF;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:Wholeserum-liquid
Immunogen:Aminoacids315-630ofthehumanprotein

Theproteinprovedtobeanunusualmemberoftheintermediatefilamentfamily,containinganα-helicalregionhomologoustothatfoundinkeratinandneurofilamentsubunits.TheHumanGenomeOrganization(HUGO)GeneNomenclatureCommittee(HGNC)nameforNestinisNES.
Image:SH-SY5YneuroblastomacellswerestainedwithNestin(RA221025)at1:5,000(red)andCH22108againstvimentin(green)at1:10,000.Vimentinismainpartnerofnestinformingheterdimersandfurtherpolymerizingtoformintermediatefilaments.Co-localizationofvimentinandnestinincellswasshowninyellow.BlueisDAPIstainingofDNA.
Nestinisrelativelypoorlyconservedinproteinsequenceacrossspeciesboundaries,sothatthemouseandhumanproteinshaveanoverallidentityofonly62%.Asaresultantibodiestothehumanproteinoftenfailtorecognizetherodenthomologueandviceversa.Antibodiestonestinarewidelyusedtoidentifyneuralstemcells.Thisantibodywasraisedinrabbitagainstrecombinanthumannestinfromaa315-630,whichhasonly55%identitytohomologousmousesequence.Nestin(RA22125)thereforerecognizesnestinproteininhuman,butnotinrodent.