
Type:ChickenIgY
Applications:ICC;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Peptidecommontomouse(NP_035831)andhuman(NP_003371)Vimentin

Humanvimentinisa53,521daltonprotein(466aminoacids)expressedinmanycelltypesofmesodermalorigin,andisoneoftheintermediatefilamenttypesthatformtheCytoskeleton.Intheembryonicnervoussystem,however,vimentinisexpressedbymigratingneuralcrestcellsformingthePNS,andbyneuralstemcellsoftheCNS.Insomeformsofglioblastoma,vimentinisre-expressedbythetumorcellsandservesasageneralindicatoroftheirundifferentiatedstate.
ImmunohistochemicalstainingforVimentinisalsocharacteristicofsarcomas(ofneural,muscleandfibroblastorigin)comparedtocarcinomaswhicharegenerallynegative.Melanomas,lymphomasandvasculartumorsmayallstainforVimentin.Vimentinantibodiesarethusofvalueinthedifferentialdiagnosisofundifferentiatedneoplasmsandmalignanttumors.
Image:cultureddissociatedcellsfromane13mousebrain),vimentin(greenstaining)canbeseenformingthecytoskeletonofimmatureastrocyteProgenitorcells.DAPI(bluestaining)allowsvisualizationofnuclei.
Vimentin:CustomerPublications