
Type:RabbitIgG
Applications:IHC
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M;Pr;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid

Endomorphinsarerecentlydiscoveredendogenousopioidpeptidescomposedoffouraminoacids:Endomorphin-1(EM-1)isTyr-Pro-Trp-Phe-NH2,and Endomorphin-2(EM-2)isTyr-Pro-Phe-Phe-NH2.Thesepeptideshaveaveryhighaffinityandspecificityformu-opioidreceptorsasithasbeenreportedinmanypharmacologicalstudies.EM-1isdistributedinmanybrainregions,whereasEM-2isprevalentinbrainstemandspinalcord. EM-1andEM-2exerttheirantinociceptiveactivitybyinteractingwithmu-opioidreceptors.EM-1anfEM-2bindwithmu-opioidreceptorwithhighaffinityandselectivityincomparisonwithbindingtoopioiddeltaandkappareceptors.EM-1andEM-2tetrapeptidesactivateG-proteinsandinhibitadenylylcyclaseactivitywhenbindtomu-opioidreceptors.
Image:Endomorphin-1and2staininginratparaventricularthalamicnucleus.
Note:Ouraffinity-purified anti-END-1antibody antibodylabelsneuronalfibersinvariousbrainregionsincluding(butnotlimitedto)thalamicnuclei,periaqueductalgraymatter,amygdala,locuscoeruleus,nucleusaccumbens,parabrachialnucleus,nucleustractussolitariusandseptum.Ouraffinity-purifiedanti labelsanti-END-2 antibodiy neuronalprocessesinspinalcorddorsalhornandtrigeminaltract,andlabelneuronalcellbodiesinthalamus.EM-1and2 distributioninbrainoverlapswithmu-opioidreceptors.