Type:ChickenIgY
Applications:ICC;IF;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:B;H;M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Full-lengthrecombinanthumanprotein.HGNCnameforthisproteinisCALB1.
Calbindin,alsoknownascalbindin1orcalbindin-D28kisamemberofthelargesuperfamilyofcytoplasmicCa2+bindingproteins.Calbindin-1belongstothesubclassofthesecontainingthe"EFhand"Ca2+bindingmotiforiginallycharacterizedinparvalbumin.Calbindinisexpressedinmammalianbrain,intestine,kidneyandpancreas.Inthebrainitislocalizedincertainclassesofneurons,andantibodiestoitareusefulforidentifyingspecificneuronalcelltypes.ItisparticularlyconcentratedinthedendritesandperikaryaofcerebellarPurkinjecells,butisalsofoundinmanyGABAergicinterneuronsinthecortex.
Images:Adultratbraincortex(Left)andstriatum(Right)sections(45μM;fixedbytranscardialperfusionwith4%paraformaldehyde)werestainedwithourCalbindin(1:1,000,red),andourmonoclonalmouseFox3/NeuN(MO22112).Calbindinlabelsasubsetofsparsely-distributedinterneurons(calbindin-positvieinterneurons)inthecortex(Left),andmoredensely-distributedneuronsinthestriatum(Right).SinceneuronsalsoexpressFox3/NeuN,calbindin-positivecellsappeartobegoldtoyellow.Insetsarehighmagnificationimagesofboxedareaofeachimage.BlueisDapinucleusstaining.
Thefunctionofcalbindin-1appearstobeprimarilybufferingtheCa2+ levelincells.TheaffinityofcalbindinforCa2+ islowatthetypicalrestingcytoplasmicCa2+ levelofaround100nM,andtheproteinonlybindsCa2+ significantlywhenlevelsincreasegreatly.Accordingly,itiswidelythoughtthattheprimaryfunctionofthisproteinistoactasaCa2+ buffer.BufferingCa2+ isimportant,asuncontrolledincreasesinthelevelofthiscationcanleadtobothapoptosisduetoCa2+stimulatedreleaseofproteinsfrommitochondriaandnecrosisduetotheactivationofCa2+dependentproteases.
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Calbindin-Mouse-Cat#:MO22146 Calbindin-Mouse-Cat#:MO20016 CalbindinD CalciumSignalingAntibodies | AllNeurotransmissionResearchAntibodies Neuron/SynapseMarkers PainandInflammation | NeurodegenerativeDiseaseResearchAntibodies NeurodegenerativeDiseaseResearchProteins PrimaryNeuronsandAstrocytes-Primaryhuman,ratandmouseneuronsandastrocytes |
Image:WesternblotanalysisofCalbindin.Blotsofratbrainlysate(left),0.5µgofHis-taggedrecombinantproteins(right)wereprobedwithCalbindinat1:5,000.Lane1:Parvalbumin,Lane2:Calretinin,Lane3:Calbindin.Inratbrainlysates,thisantibodyrecognizesaclearbandat30kDawhichrepresentscalbindinanditreactsonlywithcalbindinprotein,andnotothercalcium-bindingproteins.Thebandat~60kDaismostlikelythedimerofcalbindin.
BelowImage,courtesyofKatherineGiven,UniversityofColorado.Protocol:Ex-vivocerebellarsliceswerefixedin4%PFAfor20min.ForIHC,sectionswerepermeABIlizedin10%TritoninPBSfor20min,rinsedinPBSandthenblockedin5%NDSinPBSTx(0.3%).PrimariesweapplyRTovernightin5%NDS.WeusedNeuromicsCknCalbindin(CH22118)at1:1000(green),combinedwithMBP(red)andSox10(blue).
