Type:ChickenIgY
Applications:ICC;IF;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:liquid
Immunogen:Full-lengthrecombinanthumanprotein
CalretininisamemberofthelargesuperfamilyofcytoplasmicCa2+ bindingproteins,thusbelongstothesubclassofthesecontainingthe“EFhand”Ca2+ bindingmotiforiginallycharacterizedin parvalbumin.Calretininisexpressedinmammaliancentralnervesystem,testis,fallopiantubeandpancreas.Inthebrainitislocalizedincertainclassesofneurons,andantibodiestoitareusefulforidentifyingspecificneuronalcelltypes.Itisparticularlyconcentratedinsomecerebellargranularcellsandtheirparallelfibers,butisalsofoundinmanyGABAergicinterneuronsinthecortex.TheseGABAergicinterneurons,inmostcases,expressonlyoneofthreeCa2+ bindingproteins,namelycalretinin,calbindinorparvalbumin.Asaresult,theseimportantinhibitoryinterneuronscanbeidentifiedandsubclassifiedbasedontheircontentofthesethreeproteins.Eachtypeofneuronasdefinedinthisfashionhasparticularelectrophysiologicalandfunctionalproperties.Forexample,calbindinpositiveinterneuronsarenotfast-spikingasareparvalbuminexpressinginterneurons.Humancalretininisalsoknownas29kDacalbindinandcalbindin-2,foritssequenceisrelatedtocalbindin.
Image:(left) Adultratbrainhippocampussection(45μM;fixedbytranscardialperfusionwith4%paraformaldehyde)wasstainedwithCH22116(1:1,000,red),andourrabbitanti-MeCP2(RA22123green).Calretininlabelsasubsetofhippocampalinterneurons,whichalsoexpressMeCP2inthenucleustogiveayellowcolor.(Right) Adultratcortexsectionwasco-stainedwithCalretinin(CH22116;red)andourmouseanti-calbindinantibody(MO22146;green).Eachantibodyspecificallylabelsasubsetofinterneurons(i.e.,calretinin-positiveorcalbindin-postive)thatexpresseachMarkerexclusively.Insetsarehigh-magnificationimages oftheboxedareaineachpicture.BlueisDapistainingthatlabelsDNA.
CalretinincontainssixEF-handdomains.FourofthembindCa2+ withhighaffinityinacooperativemanner,onewithlowaffinityandthelastoneisnon-functional,withoutCa2+-bindingABIlity.ThefunctionofcalretininappearstobeprimarilybufferingtheCa2+ levelincellsandaffectintracellularcalciumsignals.Calretinindeficiencyinmossycellsofthedentategyrusandgranulecellsresultsinabnormalexcitabilityinthecerebellarneuronalnetworkandimpairmentoflong-termpotentiationandmotorcoordination.The HGNC nameforthisproteinis CALB2.
Thisantibodywasraisedagainsthumancalretininproteinexpressedinandpurifiedfrom E.coli.Thisantibodydoesnotcross-reactwiththerelatedcalciumbindingproteinscalbindinandparvalbumin(seeBlotimage).
