
Type:MouseIgG
Applications:IHC;WB;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:HybridomaresultingfromthefusionofamousemyelomawithBcellsobtainedfromamouseimmunizedwithpurified,E.coli-derivedrecombinanthumanGlialFibrillaryAcidicProtein(rhGFAP;aa292-432;Accession#P14136)..

Thisantibodyreactswiththe52kDintermediatefilamentprotein GFAPinbrainandspinalcord.ItlabelssomeastrocytesandsomeCNSependymalcellsbutnotoligodendrocytesorneurons.Thisantibodydoesnotreactwithotherintermediatefilamentproteins
Image:Ratcorticalstemcellsweredifferentiatedfor7daysandstainedwithGFAP(red)andTuj-1 (green).ThenucleiwerestainedwithDAPI(blue)
ImmunohistochemistryProtocol:Thisantibodywasusedataconcentrationof5-10μg/mLtodetecthumanGFAPinastrocytesdifferentiatedfromneuralProgenitors.CellswerefixedwithPBScontaining4%paraformaldehydefor20minutesatroomtemperatureandblockedwithPBScontaining10%normaldonkeyserum,0.3%TritonX-100,and1%BSAfor45minutesatroomtemperature.Afterblocking,cellswereincubatedwithdilutedprimaryantibodyovernightat4°CfollowedbyRhodamineRed™-coupledanti-mouseIgGatroomtemperatureinthedarkforonehour.Betweeneachstep,cellswerewashedwithPBScontaining0.1%BSA.GFAP:CustomerPublications