
Type:ChickenIgY
Applications:ICC;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M;Pr;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:liquid
Immunogen:PurifiedbovinespinalcordGFAP

Glialfibrillaryacidicprotein(GFAP)isthe10nmorintermediatefilamentproteinfoundspecificallyinastrocyticcellsinthecentralnervoussystem.
GFAPisstronglyandspecificallyexpressedinastrocytesandcertainotherastrogliainthecentralnervoussystem,insatellitecellsinperipheralganglia,andinnon-myelinatingSchwanncellsinperipheralnerves.InmanydamageanddiseasestatesGFAPexpressionisheavilyupregulatedinastrocytes.InadditionneuralstemcellsfrequentlystronglyexpressGFAP.AntibodiestoGFAParethereforeveryusefulasMarkersofastrocyticcellsandneuralstemcells.
ImageofGFAP(red)stainingofastrocytesinprimaryculture (DAPI,blue).
GFAP:CustomerPublications
PreparationNotes:MadewithRecombinantGFAPexpressedinbacteriaandhighlypurified.SubsequentboostswereperformedwithGFAPpurifiedfromaTritonX-100extractofmyelinassociatedmaterialfrombovinespinalcord,followinganaxonalflotationprocedure(3).TheGFAPwasfurtherpurifiedbycentrifugationandionexchangechromatographyin6mureaonDEAEcellulose.