
Type:GoatIgG
Applications:IHC;WB;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:E.coli-derived,recombinanthumanSRY(sexdeterminingregionY)HMGbox1(rhSOX1;aa242-379;Accession#NP_005977withL276Isubstitution)fusedatitsN-terminuswithapeptidethataidsexpression.

TheSOX(SRY-boxcontaininggene)genefamilyencodesagroupoftranscriptionfactorsdefinedbytheconservedhighmotilitygroup(HMG)DNA-bindingdomain.Theyareinvolvedintheregulationofembryonicdevelopmentandinthedeterminationofcellfate.
SOX-1isa39kDatranscriptionfactorthatbelongstotheSOXB1subgroup.WithinthedevelopingCNS,SOX1maintainsneuralcellsinanundifferentiatedstateandhasbeenusedasaMarkerforneuralstemcells.HumanandmouseSOX1share97%aminoacidsequenceidentity.
Note:ThisantibodyhasbeenselectedforitsABIlitytorecognizehumanSOX1inIHC,directELISAsandwesternblots. Itshowslessthan2%cross-reactivitywithrhSOX2,-3,-5,-6,-7,-9,-10,-11,-15,-17and-21.
Image:SOX1 stainingof humanNTERA-2cells,aneuronalprecursorcellline,Cellswerestainedusingrhodaminered-conjugateddonkeyanti-goatsecondaryantibodyandcounterstainedwithDAPI(inset).