Type:RabbitIgG
Applications:IP;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:SyntheticphosphopeptidecorrespondingtoresiduesurroundingTyr490ofhumanTrkA
100%homologywithhumanandmouse.
TheBIOLOGicalactivitiesoftheneurotrophinsaremediatedbybindingtothedifferentmembersoftheTrkfamilytyrosinekinasereceptors.ThreeTrkfamilyproteins,TrkA,TrkBandTrkC,exhibitingdifferentligandspecificities,havebeenidentified.Attheproteinsequencelevel,Trksarehighlyconservedbetweenspeciesandtheproteinsexhibitcross-speciesactivity.
Specificity/Sensitivity:Phospho-TrkA(Tyr490)AntibodydetectsendogenouslevelsofTrkonlywhenphosphorylatedattyrosine490.ThisantibodyalsodetectsTrkBandTrkCwhenphosphorylatedatthecorrespondingresidues.
Images:IncreasedmobilizationoftrkAtoneuronalmembraneenhancesresponsetoNGFchallenge.A,Fluorescencephotomicrographsdepictingrelativelevelsofactivated/phosphorylatedtrkA(phospho-trkA)detectedunderpermeABIlizingconditionsinrepresentativeadultDRGneuronsexposedtoacidic(pH6.5)orcontrol(pH7.4)media(inthepresenceofanti-BDNF)for30min,followedbya15minchallengewithpH-specificmedium+anti-BDNFaloneorwithanti-BDNF+50ng/mlNGF.Scalebar,10μm.Scatterplotsillustratetherelationshipbetweenphospho-trkA-likelabelingintensity(y-axis;normalizedtothemeansignalintensityfromthecontrolpHgroup)andperikaryaldiameter(x-axis)forallsensoryneuronsanalyzedfromthreeseparateexperimentsexposedtocontrol(pH7.4,B)oracidic(pH6.5,C)mediaasaboveandthenchallengedwith50ng/mlNGF(opencircles)ornot(filledcircles).D,SummarybargraphdepictingrelativelevelsofactivatedtrkA(phospho-trkA;normalizedtopH7.4control)inrepresentativeadultDRGneuronsexposedtoacidic(pH6.5)orcontrol(pH7.4)mediafor30minandthenchallengedwith50ng/mlNGFandasnormalizedtothemeansignalintensityfromthecontrolpHgroup.E,FluorescencephotomicrographsofsensoryneuronsexposedtoidenticalconditionsasinAandprocessedtodetectactivated/phosphorylatedp38MAPKNote:Asignificantincreaseisobservedinthelevelsofphospho-trkAdetectedinsensoryneuronsinresponsetoacidosiswithaparallelresponseobservedforphospho-p38MAPK(E)(one-wayANOVAwithposthocTukey"s;***p<0.001;n=131–210neuronsanalyzedperexperimentalcondition).Scalebar,20μm.TheJournalofNeuroscience,8May2013,33(19):8202-8215;doi:10.1523/JNEUROSCI.4408-12.2013-phospho-TrkACustomerPublications.
