
Type:Protein
SpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllThedisulfidelinkedhomodimericproteinsvascularendothelialgrowthfactor(VEGF)andplacentagrowthfactor(PlGF)arebothmembersoftheVEGFfamilyofgrowthfactors.VEGFandPlGFproteinsshareprimarystructuralaswellaslimitedaminoacidsequencehomologywiththeAandBchainsofPDGF.Alleightcysteineresiduesinvolvedinintraandinterchaindisulfidesareconservedamongthesegrowthfactors.IntheirPDGFlikeregions,VEGFandPlGFalsoshareapproximately53%aminoacidsequencesimilarity.E.coliexpressedmonomersofPlGFandVEGFcanberefoldedinvitrotoformPlGF/VEGFheterodimers.ThepurifiedrecombinantVEGF/PlGFheterodimersandVEGFhomodimershavepotentmitogeniceffectsonendothelialcells.However,VEGF/PlGFheterodimersdisplayapproximately50foldlessmitogenicactivitythanVEGFhomodimers.Incontrast,PlGFhomodimershavelittleornomitogeniceffectonendothelialcells.ThepresenceofnaturalVEGF/PlGFheterodimersintheconditionedmediaofanumberofhumantumorcelllineshasbeendemonstratedusingaVEGF/PlGFheterodimerspecificELISA.